A multiplex PCR assay for determination of mating type in isolates of the honey bee fungal pathogen, Ascosphaera apis
نویسندگان
چکیده
A multiplex PCR assay for determination of mating type in isolates of the honey bee fungal pathogen, Ascosphaera apis Kate Aronstein & Deanna Colby To cite this article: Kate Aronstein & Deanna Colby (2016): A multiplex PCR assay for determination of mating type in isolates of the honey bee fungal pathogen, Ascosphaera apis, Journal of Apicultural Research, DOI: 10.1080/00218839.2015.1109917 To link to this article: http://dx.doi.org/10.1080/00218839.2015.1109917
منابع مشابه
Standard methods for fungal brood disease research.
Chalkbrood and stonebrood are two fungal diseases associated with honey bee brood. Chalkbrood, caused by Ascosphaera apis, is a common and widespread disease that can result in severe reduction of emerging worker bees and thus overall colony productivity. Stonebrood is caused by Aspergillus spp. that are rarely observed, so the impact on colony health is not very well understood. A major concer...
متن کاملMolecular detection of Nosema ceranae in the apiaries of Kurdistan province, Iran
Nosema disease is one of the most important diseases of adult honey bees worldwide. It is known as silent killer because there are no characteristic symptoms. The aim of the present study was to determine prevalence of Nosema species in various towns of Kurdistan province in Iran. A multiplex polymerase chain reaction (multiplex-PCR) was performed for identification of Nosema ...
متن کاملAscosphaera apis, the entomopathogenic fungus affecting larvae of native bees (Xylocopa augusti): First report in South America.
BACKGROUND Nowadays several invertebrate pollinators of crops and wild plants are in decline as result of multiple and, sometimes, unknown factors; among them, the modern agricultural practices, pests and diseases are postulated as the most important factors. Bees of the genus Xylocopa are considered effective pollinators of passion fruit crops in tropical regions, as well as important pollinat...
متن کاملFirst Detection of the Larval Chalkbrood Disease Pathogen Ascosphaera apis (Ascomycota: Eurotiomycetes: Ascosphaerales) in Adult Bumble Bees
Fungi in the genus Ascosphaera (Ascomycota: Eurotiomycetes: Ascosphaerales) cause chalkbrood disease in larvae of bees. Here, we report the first-ever detection of the fungus in adult bumble bees that were raised in captivity for studies on colony development. Wild queens of Bombus griseocollis, B. nevadensis and B. vosnesenskii were collected and maintained for establishment of nests. Queens t...
متن کاملPCR diagnostic methods for Ascosphaera infections in bees.
Fungi in the genus Ascosphaera are the causative agents of chalkbrood, a major disease affecting bee larval viability. Identification of individual Ascosphaera species based on morphological features has been difficult due to a lack of distinguishing characteristics. Most identifications are based on the size and shape of the ascomata, spore balls and conidia. Unfortunately, much overlap occurs...
متن کامل